Composite
Part:BBa_K2553002:Design
Designed by: YUHE SHEN Group: iGEM18_SDSZ_China (2018-10-04)
pLuxlacO_CDA1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1315
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1315
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1315
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1315
Illegal NgoMIV site found at 244 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 544
Illegal BsaI.rc site found at 1435
Design Notes
beware of the initiator and terminator
Source
from genome of Penaeus Monodon